Tuber magnatum Pico In Melethemata inauguralia de fungorum
generatione et propagatione: 80(1788) specimens examined:
CMI-UNIBO 1160-30 CMI-UNIBO 1208-34 CMI-UNIBO 1208-35 CMI-UNIBO
1219-36 CMI-UNIBO 1289-44
Ascocarp Morphology. Ascocarp subglobose or globose or lobed,
orifice or cavity absent, 1-6(-20) cm in diameter, yellow
or ochre or brown or red, smooth, peridium glabrous, color
of spore-bearing tissue grey or yellow or brown (hazel) or
olive, veins narrow and numerous. Peridium (253-)337-421-505(-532)
micron, outermost layers (27-)45-78-111(-146) micron, hyaline
or yellow-brown (yellowish), anatomical structure pseudoparenchimatic
with roundish cells, cell tangential dimension (3-)9-12-15(-16)
micron, cell radial dimension (3-)6-9-12(-13) micron, innermost
layers (186-)251-336-421(-479) micron, color hyaline, anatomical
structure plectenchymatic, cell tangential dimension 3-6-9(-10)
micron, cell radial dimension 3-5-7 micron, external cystidia
absent. Sterile veins 50-160 micron, plectenchimatic.
Asci ans spores Morphology. Asci globose, major axis (53-)61-69-77(-90)
micron, minor axis (26-)42-53-64(-68) micron, number of spores
per ascus 1-4, irregularly clustered.
Spores subglobose or globose, major axis (20-)22-25-28(-37)
micrometer lenght, minor axis (16-)19-21-23(-25) micrometer
lenght, color yellow or pale brown, reticulation; ornamentation
length (2-)3-4-5(-7) micron; mesh width (6-)8-10-12(-16) micron;
number of meshes along the major axis (2-)3(-4).
Distribution. Habitat temperate; Putative plant host Quercus,
or Corylus, or Ostrya, or Carpinus, or Tilia, or Populus,
or Salix.
DNA polymorphism. ITS restriction pattern with EcoRI 600-650
bp; SphI 250-300 bp, or 300-350 bp; HincII less than 50 bp,
or 550-600 bp; DNA sequence ID of the ITS1-5.8S-ITS2 regions
TMAJ2509.
Mycorrhizae morphology. Mycorrhizae emanting hyphae absent,
or rare; anatomy of emanating hyphae: clamps absent; color
of emanating hyphae hyaline; color of very tip white, or whitish,
or ochre, yellowish brown; color of older parts ochre, yellowish
brown, or brown; cystidia absent, or present; needle like;
color hyaline; intercalate swelling absent; ramification absent;
anatomy of the mantle pseudoparenchymatus with epidermoid
hyphal cells" like a puzzle".